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connie cepko  (Addgene inc)


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    Structured Review

    Addgene inc connie cepko
    Connie Cepko, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 90 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/connie+cepko/pCAG-ERT2CreERT2+(Plasmid+%2313777)/pm41912583-157-12-14
    Average 93 stars, based on 90 article reviews
    connie cepko - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Transfection:

    Article Title: Clostridioides difficile TcdB induces expression of its receptor (CSPG4) through a noncanonical Hippo signaling mechanism.
    Article Snippet: HeLa cells were transfected with the plasmid pCMV-flag YAP2 5SA, which was a gift from Kunliang Guan (Addgene plasmid # 27371; http://n2t.net/addgene:27371) (24). .. HeLa cells were also transfected with the control plasmid pCMV-GFP, which was a gift from Connie Cepko (Addgene plasmid # 11153 ; http://n2t.net/addgene:11153) (38). ..

    Article Title: Clostridioides difficile TcdB induces expression of its receptor (CSPG4) through a noncanonical Hippo signaling mechanism
    Article Snippet: HeLa cells were transfected with the plasmid pCMV-flag YAP2 5SA, which was a gift from Kunliang Guan (Addgene plasmid # 27371; http://n2t.net/addgene:27371 ) ( ). .. HeLa cells were also transfected with the control plasmid pCMV-GFP, which was a gift from Connie Cepko (Addgene plasmid # 11153; http://n2t.net/addgene:11153 ) ( ). ..

    Control:

    Article Title: Clostridioides difficile TcdB induces expression of its receptor (CSPG4) through a noncanonical Hippo signaling mechanism.
    Article Snippet: HeLa cells were transfected with the plasmid pCMV-flag YAP2 5SA, which was a gift from Kunliang Guan (Addgene plasmid # 27371; http://n2t.net/addgene:27371) (24). .. HeLa cells were also transfected with the control plasmid pCMV-GFP, which was a gift from Connie Cepko (Addgene plasmid # 11153 ; http://n2t.net/addgene:11153) (38). ..

    Article Title: Clostridioides difficile TcdB induces expression of its receptor (CSPG4) through a noncanonical Hippo signaling mechanism
    Article Snippet: HeLa cells were transfected with the plasmid pCMV-flag YAP2 5SA, which was a gift from Kunliang Guan (Addgene plasmid # 27371; http://n2t.net/addgene:27371 ) ( ). .. HeLa cells were also transfected with the control plasmid pCMV-GFP, which was a gift from Connie Cepko (Addgene plasmid # 11153; http://n2t.net/addgene:11153 ) ( ). ..

    Plasmid Preparation:

    Article Title: Clostridioides difficile TcdB induces expression of its receptor (CSPG4) through a noncanonical Hippo signaling mechanism.
    Article Snippet: HeLa cells were transfected with the plasmid pCMV-flag YAP2 5SA, which was a gift from Kunliang Guan (Addgene plasmid # 27371; http://n2t.net/addgene:27371) (24). .. HeLa cells were also transfected with the control plasmid pCMV-GFP, which was a gift from Connie Cepko (Addgene plasmid # 11153 ; http://n2t.net/addgene:11153) (38). ..

    Article Title: Surface Marker Identification to Capture Live Circulating Tumor Cells in Metastatic Triple-Negative Breast Cancer
    Article Snippet: .. Lewis (Addgene, plasmid #110834; ref. ) and pFUGW-tdtomato was a gift from Connie Cepko (Addgene, plasmid #22478; ref. ). pWPT-RFP was kindly provided by Xiang Zhang. ..

    Article Title: Adjuvant for animal and human vaccines
    Article Snippet: .. Amplified gene fragments were cloned into EcoRI and NotI restriction sites of pCAG-GFP plasmid, a gift from Connie Cepko (addgene plasmid #11150, FIG. 3A). .. To confirm insertion of genes in the correct orientation, DNA sequencing was performed at the UW-Madison Biotechnology Center with an ABI Prism 3730XL DNA analyzer using BigDye terminators (Applied Biosystems, CA).

    Article Title: Epigenetic regulation of serine biosynthesis by PHF8 during neurogenesis
    Article Snippet: The CAG-mCherry construct was a gift from Jordan Green (Addgene plasmid #108685; http://n2t.net/addgene:108685 ; RRID:Addgene_108685) (Mishra et al, ). .. The pCAG-GFP plasmid was a gift from Connie Cepko (Addgene plasmid #11150; http://n2t.net/addgene:11150 ; RRID: Addgene_11150 ) (Matsuda and Cepko, ). ..

    Article Title: CellCousin2: an optimized system for partial ablation and tracing of regenerative lineages.
    Article Snippet: The fabp10a:ERT2CreERT2; cryaa:mCerulean construct [abbreviated as fabp10a:ERCreER] was generated by replacing the CreERT2 in fabp10a:CreERT2; cryaa:mCerulean construct (Addgene plasmid # 230044 ; http://n2t.net/addgene:230044 ; RRID:Addgene_230044) 23 with ERT2CreERT2 using EcoRI/NotI. .. The ERT2CreERT2 sequence was obtained fom pCAG-ERT2CreERT2, which was a gift from Connie Cepko (Addgene plasmid # 13777 ; http://n2t.net/addgene:13777 ; RRID:Addgene_13777) 33. ..

    Article Title: Clostridioides difficile TcdB induces expression of its receptor (CSPG4) through a noncanonical Hippo signaling mechanism
    Article Snippet: HeLa cells were transfected with the plasmid pCMV-flag YAP2 5SA, which was a gift from Kunliang Guan (Addgene plasmid # 27371; http://n2t.net/addgene:27371 ) ( ). .. HeLa cells were also transfected with the control plasmid pCMV-GFP, which was a gift from Connie Cepko (Addgene plasmid # 11153; http://n2t.net/addgene:11153 ) ( ). ..

    Article Title: CKII-Phosphorylated HPV-16 E7 Disrupts Planar Cell Polarity by Recruiting Vangl1 Phospho-E7 Hijacks Vangl1 Trafficking
    Article Snippet: .. E7 fragments were subcloned into pCMV GFP plasmid, a kind gift from Connie Cepko (Addgene,11153). ..

    Amplification:

    Article Title: Adjuvant for animal and human vaccines
    Article Snippet: .. Amplified gene fragments were cloned into EcoRI and NotI restriction sites of pCAG-GFP plasmid, a gift from Connie Cepko (addgene plasmid #11150, FIG. 3A). .. To confirm insertion of genes in the correct orientation, DNA sequencing was performed at the UW-Madison Biotechnology Center with an ABI Prism 3730XL DNA analyzer using BigDye terminators (Applied Biosystems, CA).

    Clone Assay:

    Article Title: Adjuvant for animal and human vaccines
    Article Snippet: .. Amplified gene fragments were cloned into EcoRI and NotI restriction sites of pCAG-GFP plasmid, a gift from Connie Cepko (addgene plasmid #11150, FIG. 3A). .. To confirm insertion of genes in the correct orientation, DNA sequencing was performed at the UW-Madison Biotechnology Center with an ABI Prism 3730XL DNA analyzer using BigDye terminators (Applied Biosystems, CA).

    Sequencing:

    Article Title: CellCousin2: an optimized system for partial ablation and tracing of regenerative lineages.
    Article Snippet: The fabp10a:ERT2CreERT2; cryaa:mCerulean construct [abbreviated as fabp10a:ERCreER] was generated by replacing the CreERT2 in fabp10a:CreERT2; cryaa:mCerulean construct (Addgene plasmid # 230044 ; http://n2t.net/addgene:230044 ; RRID:Addgene_230044) 23 with ERT2CreERT2 using EcoRI/NotI. .. The ERT2CreERT2 sequence was obtained fom pCAG-ERT2CreERT2, which was a gift from Connie Cepko (Addgene plasmid # 13777 ; http://n2t.net/addgene:13777 ; RRID:Addgene_13777) 33. ..

    other:

    Article Title: Epigenetic regulation of serine biosynthesis by PHF8 during neurogenesis.
    Article Snippet: Antibodies used were anti: PHF8 (Abcam, ab36068), H4K20me1 (Abcam, ab9051), H3K9me2 (Abcam, ab1220), H3K9me3 (Abcam, ab8898), NFIA (Abcam, ab 228897), ULK1 (Cell Signaling, #8054), p-ULK1 (ser 757) (Cell Signaling, #6888), alpha TUBULIN (Abcam, ab4074), p-H2A.X (Ser139) clone JBW301 12 EMBO reports © The Author(s) (Merck, 05-636), IdU (BD biosciences, Cat n° 34758), CldU (Abcam, ab6326), LC3 (MBL, PM036), SQSTM1 (Progen, GP62C), S6K (Cell Signaling, #9202S), p-S6K (thr389) (Cell Signaling, #9234), B-actin (Merck, A2228), TFEB (ProteinTech, 13372-1-AP), GFP (AvesLabs GFP-1010), SOX2 (R&D Systems AF2088), TBR2 (Abcam 183991), TUJ1 (Biolegend 801202), DAPI (Thermo Fisher, D1306), and DCX (Millipore, 324385) was used at 1 μg/ml.



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    Addgene inc connie cepko
    Connie Cepko, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/connie+cepko/pCAG-ERT2CreERT2+(Plasmid+%2313777)/pm41912583-157-12-14
    Average 93 stars, based on 1 article reviews
    connie cepko - by Bioz Stars, 2026-09
    93/100 stars
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